Resolution of overlapping UV-visible absorption bands: Quantitative analysis of tautomeric equilibria

✍ Scribed by Liudmil Antonov; Stefan Stoyanov

• Book ID: 102983770
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 607 KB
• Volume: 314
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00281-4

✦ Synopsis

A quantitative approach for the analysis of two-component mixtures with unknown spectra of the pure components, based on resolution of the UV-visible absorption spectra into individual bands is proposed and its reliability is confirmed. The proposed method is applied for the analysis of the tautomeric equilibrium of 1-phenylazo-4-naphthol.

The molar parts and individual spectra of both tautomeric forms and tautomeric constant K, are determined. The proposed general approach could be applied for quantitative analysis of any other two-component equilibria including aggregation, acid-base processes, complex formation, where the absorption spectra of the individual forms cannot be obtained experimentally.