Resolution of non-protein amino acids via microbial protease-catalyzed ester hydrolysis: marked enhancement of enantioselectivity by the use of esters with longer alkyl chains and at low temperature

✍ Scribed by Toshifumi Miyazawa; Hiroe Minowa; Toyoko Miyamoto; Kiwamu Imagawa; Ryoji Yanagihara; Takashi Yamada

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 232 KB
• Volume: 8
• Category: Article
• ISSN: 0957-4166
• DOI: 10.1016/s0957-4166(97)00002-5

✦ Synopsis

In the microbial protease-catalyzed hydrolysis of amino acid esters with the free cx-amino group, the enantioselectivity can be enhanced greatly by employing esters with longer alkyl chains such as the isobutyl ester instead of the conventional methyl ester and by conducting the reaction at low temperature. (~