Resolution of multicomponent fluorescent mixtures by analysis of the excitation–emission–frequency array

✍ Scribed by Donald S. Burdick; Xin M. Tu; Linda B. McGown; David W. Millican

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 706 KB
• Volume: 4
• Category: Article
• ISSN: 0886-9383
• DOI: 10.1002/cem.1180040104

✦ Synopsis

Fluorescence lifetime provides a third independent dimension of information for the resolution of total luminescence spectra of multicomponent mixtures. The incorporation of this parameter into the excitation-emission matrix (EEM) by the phase modulation technique results in a three-dimensional excitation-emission-frequency array (EEFA). Multicomponent analysis based on the three-dimensional EEFA brings a qualitative change for the resolved spectra, i.e. individual spectra can be uniquely resolved, which is impossible with any two-dimensional analysis. In this paper we present a method for analyzing the EEFA. We show mathematically that with the three-dimensional analysis of the EEFA individual spectra and lifetimes can be obtained. Our algorithm is developed in mathematical detail and is demonstrated by its application to a two-component mixture.