Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers
✍ Scribed by D. Ballon; U. Mahmood; A. Jakubowski; J. A. Koutcher
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 395 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
A technique is described which reduced the magnetic susceptibility induced line broadening in NMR spectra obtained from three biological systems at 4.7 Tesla. Proton spectra from a sealed suspension of HL60 leukemic myeioblasts yielded minimum linewidths of 1.3 Hz at 200 MHz (0.0065 ppm) after 10 min of automated shimming. ^31^P spectra from an in vivo murine MCa mammary carcinoma yielded a well‐resolved phospharylcholine resonance without proton decoupling and with the resistive shim coil currents set to zero. ^31^P spectra from a perfused suspension of RIF‐1 fibrosarcoma cells exhibited a γ‐nucleoside triphosphate resonance which was resolved into purine and pyrimidine components.