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Resolution and contrast in confocal optical scanning microscopes

✍ Scribed by A.M. Hamed

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
349 KB
Volume
16
Category
Article
ISSN
0030-3992

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Spatial resolution and the sensitivity to detect a fluorophore are the two most important optical parameters that characterize a confocal microscope. However, these are rather difficult to estimate quantitatively. We show that fluorescence correlation spectroscopy (FCS) provides an easy and reliable