Resolubilization of TCA precipitated plant proteins for 2-D electrophoresis

A competent extraction method of plant p

A competent extraction method of plant proteins for 2-D gel electrophoresis

✍ Ahmed Ashoub; Thomas Berberich; Tobias Beckhaus; Wolfgang Brüggemann 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 119 KB

Profiling of host cell proteins by two-d

Profiling of host cell proteins by two-dimensional difference gel electrophoresis (2D-DIGE): Implications for downstream process development

✍ Mi Jin; Nicolas Szapiel; Jennifer Zhang; John Hickey; Sanchayita Ghose 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 526 KB

## Abstract Host cell proteins (HCPs) constitute a major group of impurities for biologic drugs produced using cell culture technology. HCPs are required to be closely monitored and adequately removed in the downstream process. However, HCPs are a complex mixture of proteins with significantly dive

Micro-high-performance liquid chromatogr

Micro-high-performance liquid chromatography platform for the depletion of high-abundance proteins and subsequent on-line concentration/capturing of medium and low-abundance proteins from serum. Application to profiling of protein expression in healthy and osteoarthritis sera by 2-D gel electrophoresis

✍ Yazen Jmeian; Ziad El Rassi 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 447 KB 👁 2 views

Micro-high-performance liquid chromatogr

Micro-high-performance liquid chromatography platform for the depletion of high-abundance proteins and subsequent on-line concentration/capturing of medium and low-abundance proteins from serum. Application to profiling of protein expression in healthy and osteoarthritis sera by 2-D gel electrophoresis

✍ Yazen Jmeian; Ziad El Rassi 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 128 KB 👁 2 views