Resin comparison and fast automated stepwise conventional synthesis of human SDF-1α

✍ Scribed by Hirendra Patel; Christina A. Chantell; German Fuentes; Mahendra Menakuru; Jae H. Park

• Book ID: 105359692
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 163 KB
• Volume: 14
• Category: Article
• ISSN: 1075-2617
• DOI: 10.1002/psc.1056

✦ Synopsis

Abstract

Human SDF‐1α contains 68 amino acids and is a member of the chemokine family of peptides. This long peptide was synthesized stepwise using classical conditions in 101 h. The reaction times were then reduced to deprotection times of 2 × 2 min and coupling times of 2 × 2.5 min, resulting in a total synthesis time of 22 h. The effect of different resins, resin substitutions and deprotection reagents on the crude peptide purities was compared. A small portion of crude peptide was purified using an RP‐HPLC column and the mass of the final product was confirmed with MALDI‐TOF mass spectrometry. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd.