Rescue of SV40 following transfection of TC7 cells with cellular DNAs containing complete and partial SV40 genomes
✍ Scribed by Lipotich, George ;Moyer, Mary P. ;Moyer, Rex C.
- Book ID
- 104769341
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 378 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
✦ Synopsis
Infectious SV40 virions could be rescued from permissive TC7 cells within one to three subcultures following cotransfection with two cellular DNAs, each containing a complementary portion of the SV40 genome. SV40 virions could also be rescued by transfection of TC7 cells with cellular DNAs from a variety of SV40 transformed cells containing complete genome equivalents but not from cells containing subgenomes alone or defective genomes. Infectious virus was not rescued if the transfecting DNA species was treated with DNAase or if the DEAE-dextran pretreatment of the recipient cells was omitted.