Requirements for growth of human erythroid progenitors in nutrient agar
โ Scribed by E. Januszewicz; I. A. Cooper; T. R. Bradley
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 493 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0361-8609
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Erythroid progenitors from normal human marrow were purified by a two-step immune panning method permitting both the enrichment of erythroid progenitors (plating efficency up to 10%) and the separation of CFU-E from BFU-E. The purified erythroid progenitors wcre grown in serum-replaced conditions; i
## Abstract Assay of red cell progenitors by colony formation in culture is expected to allow study of early events in erythroid differentiation in animal models and in man. In this communication, a simplification of the culture method for mouse bone marrow cells originally reported by Stephenson e