Reproductive cloning conserves cellular senescence
β Scribed by Sedivy, John M.
- Book ID
- 109911667
- Publisher
- Nature Publishing Group
- Year
- 2003
- Tongue
- English
- Weight
- 124 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1465-7392
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π SIMILAR VOLUMES
By using differential display, we cloned the human counterpart of the murine gene Hic-5 from senescent human keratinocytes. The full-length cDNA contained a short GC-stretch proceeding a consensus Kozak sequence followed by a single open reading frame of 1338 bp encoding a 461Β±amino acid protein wit
We previously reported an efficient method for subtractive cDNA cloning using oligo(dT) \()_{30}\)-Latex and polymerase chain reaction (PCR) (E. Hara et al., \(\mathrm{Nu}\) cleic Acids Res. 19, 7097-7104, 1991). The subtraction was performed by hybridization between mRNA of cell type \(B\) and the