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Reply to the Letter by Dr L. Filik

✍ Scribed by Elias Kouroumalis; Argyro Voumvouraki

Book ID: 114727645
Publisher: John Wiley and Sons
Year: 2012
Tongue: English
Weight: 29 KB
Volume: 42
Category: Article
ISSN: 0014-2972
DOI: 10.1111/j.1365-2362.2012.02690.x

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Dr. Vinogradov points out that propidium iodide (PI) binding data do not always reflect accurate genome size values. PI binding can be influenced by the chromatin conformation. In addition, PI is not DNA specific and pretreatment of samples with RNase can affect the fluorescence intensity in an unpr