Reply to the letter by Dr. Vinogradov
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R. Capparelli; C. Cottone; L. D'Apice; M. Viscardi; D. Iannelli
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Article
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1999
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John Wiley and Sons
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English
โ 106 KB
๐ 2 views
Dr. Vinogradov points out that propidium iodide (PI) binding data do not always reflect accurate genome size values. PI binding can be influenced by the chromatin conformation. In addition, PI is not DNA specific and pretreatment of samples with RNase can affect the fluorescence intensity in an unpr