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Reply to Dr. Stellar's letter

โœ Scribed by Semion Rochkind


Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
140 KB
Volume
8
Category
Article
ISSN
0196-8092

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โœฆ Synopsis


Reply to Dr. Stellar's Letter

We would like to take this opportunity to comment on a number of points raised in Dr. Stellar's letter, which we feel are in need of clarification. This we shall do in the order they appear in his letter.

The measurements of energy actually reaching the nerve in the rats were mentioned in a previous work [Nissan et al., 19861. We found that laser irradiation penetrates through skin and muscles, and 3-10% of the energy reaches the sciatic nerve.


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Dr. Vinogradov points out that propidium iodide (PI) binding data do not always reflect accurate genome size values. PI binding can be influenced by the chromatin conformation. In addition, PI is not DNA specific and pretreatment of samples with RNase can affect the fluorescence intensity in an unpr