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Reply to comments by Dr V. Babrauskas

✍ Scribed by Herman H. Forsten

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
36 KB
Volume
22
Category
Article
ISSN
0308-0501

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Dr. Vinogradov points out that propidium iodide (PI) binding data do not always reflect accurate genome size values. PI binding can be influenced by the chromatin conformation. In addition, PI is not DNA specific and pretreatment of samples with RNase can affect the fluorescence intensity in an unpr