Mutagenesis by apurinic sites in normal
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Donna K. Klinedinst; Norman R. Drinkwater
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Article
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1992
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John Wiley and Sons
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English
β 1019 KB
## Abstract We used a shuttle vector based on the EpsteinβBarr virus origin of plasmid replication (oriP) to determine the types of mutations induced by depurination in human cells. Plasmid DNA was incubated at pH 2 at 40Β°C for various times to induce up to 20 apurinic (AP) sites per 9.7βkb plasmid