## Abstract Decreasing replicative potential and dedifferentiation of articular chondrocytes during expansion in cell culture are essential limitations for tissue engineering and cell therapy approaches. Telomeres and telomerase play a key role in cell development, aging, and tumorigenesis. There i
Replicative aging of human articular chondrocytes during ex vivo expansion
✍ Scribed by Dominik Parsch; Tim H. Brümmendorf; Wiltrud Richter; Jörg Fellenberg
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 233 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0004-3591
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## Background: Genetically engineered chondrocytes could be used to enhance cartilage repair. fibroblast growth factor 2 (fgf-2) is a mitogen for chondrocytes and may be a candidate for gene transfer approaches to stimulate chondrocyte proliferation. in the present study, we tested the hypothesis t
Adult human articular chondrocytes were expanded in a medium with 10% serum (CTR) or further supplemented with different mitogens (i.e., EGF, PDGFbb, FGF-2, TGFb1, or FGF-2/TGFb1). Cells were then induced to redifferentiate in 3D pellets using serum-supplemented medium (SSM), serum-free medium (SFM)