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Replication of small plasmids in extracts of Escherichia coli

✍ Scribed by Staudenbauer, Walter L.


Book ID
118287484
Publisher
Springer
Year
1976
Tongue
English
Weight
758 KB
Volume
145
Category
Article
ISSN
0026-8925

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πŸ“œ SIMILAR VOLUMES


Replication of small plasmids in extract
✍ Staudenbauer, Walter L. πŸ“‚ Article πŸ“… 1976 πŸ› Springer 🌐 English βš– 782 KB

The role of the three E. coli DNA polymerases (pol I, II, and III) in the replication of Col E1 DNA and other small plasmids with similar replicative properties was investigated in a soluble in vitro system prepared by freeze-thaw lysis of chloramphenicol-treated cells (Staudenbauer, 1976). Extracts

Asymmetric replication of an oriC plasmi
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Plasmid pTSO118 containing the Escherichia coli origin of replication, oriC, initiated replication simultaneously with the chromosome when temperature-sensitive host cells were synchronized by temperature shifts. Replicating intermediates of the plasmid as well as of the chromosome were isolated fro

Origin of replication of Escherichia col
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The nucleotide sequence of a region of plasmid RSF 1030 that includes the origin of DNA replication was determined using the DNA of a small derivative, pST19. The nucleotide sequence of the pST 19 origin region is very similar to that of the ColE1 origin except for a 25 base pair (bp) deletion about