Replication of bacteriophage lambda DNA
β Scribed by Enquist, L.W.; Skalka, A.M.
- Book ID
- 121777237
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 506 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0968-0004
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A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/
We show that a collection of 93 E. coli mutations which map between thr and leu and which block phage lambda DNA replication define two closely linked cistrons. Work published in the accompanying paper shows that these mutations also affect host DNA replication, so we designate them dnaJ and dnaK; t