Repetitive somatic embryogenesis in peanut cotyledon cultures by continual exposure to 2,4-d
✍ Scribed by Charleen M. Baker; Hazel Y. Wetzstein
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 439 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
✦ Synopsis
Somatic embryos from immature cotyledons in peanut (Arachis hypogaea) were initiated on media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Over 90% primary embryogenesis and 41--46% repetitive embryogenesis were obtained 12 weeks after initiation by maintaining embryogenic cultures on medium containing 20 mg 1-1 2,4-D. Maintenance of cultures on medium with 30 or 40 mg 1-l 2,4-D resulted in lower primary and secondary embryogenesis, and proliferation of nonembryogenic callus. Transfer of embryogenic cultures to a secondary medium with 10 or 20 mg I-~ 2,4-D significantly enhanced secondary embryogenesis compared to basal medium without the growth regulator. The use of Murashige & Skoog versus Finer's media had no significant effect on embryogenesis (85-95%), repetitive embryogenesis (11-37%) or mean embryo number. Secondary embryogenesis was also maintained for over one year by repeated subculture of isolated somatic embryos on medium with 20 mg 1-l 2,4-D.