Repetition structure of mammalian nuclear DNA

✍ Scribed by Frazer Smillie; William Bains

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 358 KB
• Volume: 142
• Category: Article
• ISSN: 0022-5193
• DOI: 10.1016/s0022-5193(05)80102-x

✦ Synopsis

We have used Fragmentation Sequencing logic to analyse the repetition structure of several large human genomic genes; The method, based on a proposed laboratory scheme for DNA sequencing, detects short sequences which are repeated near, but not necessarily adjacent, to each other (cryptically simple DNA). We find a low frequency of such repeats. There is a slight excess of such repeats in introns over exons, and a slight but significant excess in genomic DNA over random DNA, confirming that cryptically simple sequences are over-represented in the genome. The analysis suggests that Fragmentation Sequencing will be a suitable method for sequencing large mammalian genes.