Repeatability of a reference region model for analysis of murine DCE-MRI data at 7T

✍ Scribed by Thomas E. Yankeelov; Laura M. DeBusk; D. Dean Billheimer; Jeffrey J. Luci; P. Charles Lin; Ronald R. Price; John C. Gore

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 457 KB
• Volume: 24
• Category: Article
• ISSN: 1053-1807
• DOI: 10.1002/jmri.20729

✦ Synopsis

Abstract

Purpose

To test the repeatability of a reference region (RR) model for the analysis of dynamic contrast‐enhanced MRI (DCE‐MRI) in a mouse model of cancer at high field.

Materials and Methods

Seven mice were injected with 10^6^ 4T1 mammary carcinoma cells and imaged eight to 10 days later on a Varian 7.0T scanner. Two DCE‐MRI studies were performed for each mouse (separated by 2.5 hours). The RR model was used to analyze the data, and returned estimates on the perfusion‐permeability index (K^trans^) for the RR and the tissue of interest (TOI), as well as the extravascular extracellular volume fraction (v~e~) for the TOI.

Results

When the first injection was compared with the second injection, all parameters tested were highly correlated (r^2^ = 0.90, 0.62, 0.82 for the RR K^trans^, TOI K^trans^, and TOI v~e~, respectively, with P < 0.001 for all). To observe a statistically significant change (at the 5% level) in a treatment study with seven animals in each group, log~10~ changes of 0.084 and 0.077 in the tumor K^trans^ and v~e~, respectively, are required.

Conclusion

If a reliable arterial input function (AIF) is unavailable, the RR model is a reasonable alternative to measuring MRI contrast‐agent (CA) kinetics in mouse models of cancer at high field. J. Magn. Reson. Imaging 2006. © 2006 Wiley‐Liss, Inc.