Repair of X-ray induced DNA damage measured by the comet assay in roots of Vicia faba
✍ Scribed by Gudrun Koppen; Karel Jan Angelis
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 148 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
The comet assay was used to measure DNA dam-DNA damage was removed in 2 hr, even in the age and repair in nuclei released from 1 cm root presence of 3-aminobenzamide. Complete repair ends of Vicia faba after X-ray irradiation. Irradia-was inhibited by incubation on ice (30% of DNA tion induced a linear increase of DNA content in remaining in tail) and partially by aphidicolin (13% comet tail with doses under various denaturation DNA remaining in tail). Under alkaline (0.3 M and electrophoretic conditions. The pH of the elec-NaOH) pretreatment and electrophoresis, more than trophoresis solution played the most important role 20% of detected DNA damage remained unrepaired in the detection of DNA damage. After irradiation after 2 hr of postirradiation incubation with and withwith 30 Gy of X-rays, most of the DNA damage out 3-aminobenzamide at room temperature. Aphidiwas removed during the first 20 min, even in the colin and incubation on ice inhibited the removal of presence of DNA repair inhibitors. This first, rapid DNA damage to 33% and 39% DNA, respectively. phase of DNA repair was not affected by incuba-Moreover, aphidicolin treatment attenuated the first tion on ice, but was partially blocked by 3-amino-phase of damage removal.