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Removal of Triton X-100 from aqueous solution using amberlite XAD-2

✍ Scribed by Peter S.J. Cheetham

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
381 KB
Volume
92
Category
Article
ISSN
0003-2697

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✦ Synopsis

Amberlite XAD-2 beads adsorbs the nonionic detergent Triton X-100 over a wide range of conditions with a maximum capacity of 0.475 mmolig dry weight. After treatment of protein solutions containing Triton X100 with XAD-2 no interference by Triton with Folin assays was observed. Adsorption1 of Triton X-100 was favored by a free-energy change of -835 calimol and by a positive entropy value. Adsorbed Triton could be completely desorbed and the XAD-2 regenerated with little loss in capacity by washing with propan-2-01. Removal of Triton by XAD-2 was also successful in columns or batch-wise on a pilot-plant scale.

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Two-dimensional nuclear Overhauser enhancement (2D NOESY) and two-dimensional heteronuclear Overhauser enhancement (2D HOESY) experiments were performed on Triton X-100 (TX-100-polyacrylamide (PAAM) aqueous solution at concentrations of 104 g l -1 TX-100 and 40 g l -1 PAAM. The absence of the NOESY