✦ LIBER ✦

Removal of contaminating nucleoside diphosphates from commercial preparations of uridine diphosphoglucose

✍ Scribed by William H. Outlaw Jr.; Daniel R.C. Hite; Giordano B. Fiore

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 317 KB
Volume: 171
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90129-7

No coin nor oath required. For personal study only.

✦ Synopsis

Nucleoside diphosphate contamination in commercial preparations of UDP-Glc poses potential problems in activity assays for enzymes that use this substrate. For removing these contaminants, we report a simple, inexpensive, and rapid method that obviates the need for uncertain corrections in assay calculations.

📜 SIMILAR VOLUMES

Purification of commercial preparations

Purification of commercial preparations of NADP+ from AMP contamination

✍ I.R. Nikulin; N.F. Belyaeva; Z.S. Kagan 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 396 KB

A method for purification of commercial preparations of NADP+ from AMP contamination is described. The purification procedure includes one-step anion-exchange chromatography on Dowex-1 (formate) and results in a highly purified salt-free coenzyme with a yield of 70-8096. The chromatography condition

An Efficient Method for the Preparation

An Efficient Method for the Preparation of 1′α-Branched-Chain Sugar Pyrimidine Ribonucleosides from Uridine: The First Conversion of a Natural Nucleoside into 1′-Substituted Ribonucleosides

✍ Tetsuya Kodama; Satoshi Shuto; Makoto Nomura; Akira Matsuda 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 188 KB 👁 1 views