Remote two-photon excited fluorescence sensing in a simulatd fermentation broth
✍ Scribed by Debora M. Dinkel; Fred E. Lytle
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 515 KB
- Volume
- 263
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
The feas&nhty of chrect quantltatlon of a fluorescent fermentahon product by means of two-photon excited molecular fluorescence and a tiber-optlc probe was mvestlgated The fermentation broth enwronment was simulated usmg buffered baker's yeast solutions To demonstrate that a two-photon Induced slgnal IS unperturbed by the presence of rmcroorgarusms, the fluorescence intensity from a fiied amount of fluorophore was momtored versus mcreasmg baker's yeast cell dens@ (O-60 g I-') The resultant two-photon induced signal remained relatwely constant, in comparison to a one-photon induced signal Quantltatlon na one-photon excitation wthout a fiber optic showed the characteristic Inner-filter roll-off of optically dense systems and quantltatlon became impossible at cell densltles higher than approxunately 20 g I-' The effect of nue-scattermg on the observed two-photon induced signal IS dwussed, and IS hypothesized to be the cause of a shght decline in signal wth increasing cell density