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Remodeling of the cytoskeletal lattice in denervated skeletal muscle

✍ Scribed by Sophie Boudriau; Claude H. Côté; Michel Vincent; Patricia Houle; Roland R. Tremblay; Peter A. Rogers

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
896 KB
Volume
19
Category
Article
ISSN
0148-639X

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✦ Synopsis

The effect of denervation-induced atrophy on the cytoskeletal lattice in rat fast-and slow-twitch skeletal muscle has been investigated. Immunochemical analyses and immunofluorescence microscopy experiments employing monospecific antibodies to dystrophin, desmin, and a-tubulin were carried out on intact and denervated muscles. The relative cellular content of dystrophin and desmin were reduced in the soleus muscle (slow-twitch), while significant increases were shown in the gastrocnemius muscle (fast-twitch). In both muscles, a-tubulin levels increased up to 12-fold as afunction of time compared to control values. lmmunofluorescence microscopy revealed a distinct rearrangement of the microtubule network toward a predominantly longitudinal alignment, which was accompanied by an increase in the density of the fluorescence. It is concluded that the relative increase of the three structural proteins in the fast-twitch gastrocnemius muscle may be related to the apparent resistance of this muscle type to denervation-induced atrophy. The increased a-tubulin content in denervated slow-and fast-twitch muscles could be indicative of an adaptive mechanism designed to maintain the integrity of the muscle fiber in view of eventual regenerative activities.

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