Bovine embryonic stem cell-like cell lin
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Saito, S. ;Strelchenko, N. ;Niemann, H.
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Article
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1992
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Springer-Verlag
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English
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A total of 14 microsurgically produced zona pellucida-free bovine demi-blastocysts were cultured for 3 days in tissue culture medium (TCM) 199 supplemented with 10% heat-inactivated newborn calf serum (NBCS). Developing embryos were continuously cultured in TCM 199 plus 10% NBCS on a feeder-layer of