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Reliable quantification of cell cycle-dependent mRNA abundance using fluorescence-activated cell sorting in Trypanosoma brucei

✍ Scribed by Ferdinand Bucerius; Markus Kador; Michael Boshart; Christian J. Janzen


Book ID
113803378
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
439 KB
Volume
175
Category
Article
ISSN
0166-6851

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## Abstract Flow cytometry (FCM) is useful for measuring DNA content as related to cell cycle position. We have extended this technology by developing a method that measures cellular DNA content using pro‐pidium iodide after permeabilization with lysolecithin. This technique maintains cell integrit