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Reliable determination of binding affinity and kinetics using surface plasmon resonance biosensors

✍ Scribed by Peter Schuck

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
690 KB
Volume
8
Category
Article
ISSN
0958-1669

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✦ Synopsis

Progress has been made in the identification of experimental and analytical procedures that allow for a more reliable determination of equilibrium and kinetic constants. Possible origins of the frequently observed deviations of the measured binding progress from that expected for chemical binding of pseudo-first order, and appropriate experimental controls have been proposed. Improved analytical approaches include the application of global analysis and analytical corrections for the influence of mass transport.

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