Helicobacter pylori is an important human gastrointestinal pathogenic bacterium which is believed to colonize approximately one-half of the world's population. Different strains of H. pylori possess virulence proteins for tissue colonization, host evasion and tissue damage. The bacteria display geno
Reliable automatic protein identification from matrix-assisted laser desorption/ionization mass spectrometric peptide fingerprints
โ Scribed by Peter Berndt; Uwe Hobohm; Hanno Langen
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 74 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0173-0835
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Identification of proteins using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) peptide mass fingerprinting (PMF) is a key technique in proteomics. The method is known to be sensitive as well as amenable to high-throughput operation, but the resulting identifications suffer f
Sodium dodecyl sulfate (SDS) is widely used for protein solubilization and for separation of proteins by SDS polyacrylamide gel electrophoresis (SDS-PAGE). However, SDS interferes with other techniques used for characterization of proteins, such as mass spectrometry (MS) and amino acid sequencing. I