## Communicated by Ulf Langergren Highly accurate and high-throughput SNP genotyping platforms are increasingly popular but the performance of suboptimal DNA samples remains unclear. The aim of our study was to determine the best platform, amplification technique, and loading concentration to maxi
β¦ LIBER β¦
Reliability of high-throughput genotyping of whole genome amplified DNA in SNP genotyping studies
β Scribed by Yvette Berthier-Schaad; Wen Hong Linda Kao; Josef Coresh; Lin Zhang; Roxann G. Ingersoll; Robert Stephens; Michael W. Smith
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 281 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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The promise of whole genome amplification (WGA) is that genomic DNA (gDNA) quantity will not limit molecular genetic analyses. Multiple displacement amplification (MDA) and the OmniPlex PCR-based WGA protocols were evaluated using 4 and 5 ng of input gDNA from 60 gDNA samples from three tissue sourc