Release of met-enkephalin and its modulation through acetylcholine receptors in the rabbit superior colliculus
✍ Scribed by R. -B. Illing; K. E. Nikolarakis; T. Wichmann; W. B. Spatz; K. Starke
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 382 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0014-4819
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✦ Synopsis
This report presents evidence for the depolarization-dependent release of met-enkephalin from the superior colliculus of the rabbit. Collicular tissue was placed in superfusion chambers and met-enkephalin accumulation in the superfusate was measured by radioimmunoassay. Exposure to high potassium concentrations (30 mM and 56 mM) increased met-enkephalin release. This is the fourth transmitter shown to be released from collicular tissue. Furthermore, we have obtained the first evidence that suggests that met-enkephalin release is susceptible to muscarinic modulation. While the depolarization-dependent release of met-enkephalin was depressed in the presence of atropine (1 microM), hexamethonium (100 microM) did not block the increase of met-enkephalin release induced by high potassium.