Release of cytoplasmic enzymes into culture fluid

✍ Scribed by Kiyoshi Nose; Keiko Nitta; Toshiko Takaoka; Hajim Katsuta

• Book ID: 102310217
• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 453 KB
• Volume: 84
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040840213

✦ Synopsis

Abstract

The release of enzymatic activities from cells grown in protein‐and lipid‐free synthetic media into culture fluids was investigated. Cell strains employed were the derivatives from mouse fibroblasts, rat liver parenchymal cells, rat ascites hepatoma cells and HeLa cells. Activities of acid DNase, acid RNase and alkaline phosphatase (ALP)‐I were detected in culture fluids as early as one day after renewal of medium, whereas those of β‐glucuronidase and acid phosphatase were not found. This release of enzymes was unlikely to be caused by cell disruption during cultivation.

The release of Dnase was inhibited by the addition of cycloheximide or actinbomycin D, whereas that of ALP‐I was not inhibited.