Release of calcium from intracellular stores in rat basophilic leukemia cells monitored with the fluorescent probe chlortetracycline
✍ Scribed by Gregory V. Marcotte; Paul J. Millard; Clare Fewtrell
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 954 KB
- Volume
- 142
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Relea5e of calcium from intracellular stores of rat basophilic leukemia cells was monitored using the fluorescent probe chlortetracycline. The ability of chlortetracycline to indicate release from intracellular calcium stores was initially validated. The decrease of chlortetracycline fluorescence upon antigen-stimulation was not the result of secretion of granule-associated dye or of changes in the properties of the membranes. The chlortetracycline fluorescence signal was not influenced by Ca2+ influx across the plasma membrane. Results obtained from these chlortetracycline fluorescence measurements corresponded well with 4'Ca efflux data, an indirect measurement of release of calcium from stores. Chlortetracycline was used to examine the rate of antigen-induced release of calcium from stores, the depletion o i intracellular calcium stores by EGTA, and the relationship between the antigen-stimulated release of stored calcium and exocyto5is.
Chlortetracycline was shown to be a useful qualitative indicator for the release of intracellular calcium with a relatively rapid response time.