Release of angiotensin I-converting enzyme by endothelial cells in vitro

✍ Scribed by James P. Noveral; Stephen N. Mueller; Elliot M. Levine

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 584 KB
• Volume: 131
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041310102

✦ Synopsis

Bovine fetal aortic endothelial cells cultured in serum-containing medium accumulate angiotensin I-converting enzyme (ACE) activity and also release it into t h e culture medium. Following subcultivation of a confluent culture using trypsin-EDTA, cellular ACE activity falls 50% within 8 h, but n o ACE activity is detected in t h e medium, suggesting intracellular loss of t h e enzyme activity. ACE activity reappears in both t h e cell lysate and culture medium after t h e culture becomes confluent. The rate of accumulation of ACE activity released into t h e medium is always greater than that for cellular activity. For example, 21 days following subcultivation 80-85% of the total culture activity is detected in t h e medium. Both cellular and medium-associated ACE decrease proportionately as t h e culture progresses through its in vitro lifespan.