Release of Adenosine from Human Sensitized Lung Fragments and its Effect on Antigen-induced Mediator Release

✍ Scribed by K. Konnaris; H.G.E. Lloyd; D.M. Temple

• Publisher: Elsevier
• Year: 1996
• Tongue: English
• Weight: 153 KB
• Volume: 9
• Category: Article
• ISSN: 0952-0600
• DOI: 10.1006/pulp.1996.0016

✦ Synopsis

Adenosine may play a role in asthma as a pro-inflammatory mediator. In this study, the release of adenosine from human sensitized lung fragments and its effect on antigen-induced histamine and leukotriene release has been explored. Antigen challenge increased histamine and leukotriene release five-fold but was without effect on adenosine release. In contrast, the adenosine deaminase inhibitor EHNA (10 microM) and the adenosine kinase inhibitor 5-iodotubericidin (10 microM) increased adenosine concentration 45-fold (P < or = 0.001; n = 4 patients). Of major interest was the finding that the non-selective, cell impermeant, adenosine antagonist pSPT (100 microM) decreased histamine and leukotriene release by 25% (P < or = 0.001) and 40%, respectively (P < or = 0.05; n = 9 patients). Additionally, the non-selective adenosine agonist NECA (10 microM) markedly inhibited antigen-induced leukotriene release by 80-90% (P < or = 0.001) and marginally inhibited histamine release by approximately 10% (P < or = 0.05; n = 9); the A2a-selective agonist DPMA (10 microM) was without effect on either histamine or leukotriene release. These results are consistent with adenosine having a biphasic effect on antigen-induced mediator release with low concentrations potentiating release and high concentrations inhibiting release. The overall stimulatory effect of endogenous adenosine supports the proposal that adenosine may act as a pro-inflammatory mediator in asthma.