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Release Kinetics and in vitro Bioactivity of Basic Fibroblast Growth Factor: Effect of the Thickness of Fibrous Matrices

✍ Scribed by Min Sup Kim; Young Min Shin; Ji-hye Lee; Sun I. Kim; Young Soo Nam; Choongsoo S. Shin; Heungsoo Shin

Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 707 KB
Volume: 11
Category: Article
ISSN: 1616-5187
DOI: 10.1002/mabi.201000222

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In this study, we fabricated non‐woven matrices using blends of polycaprolactone and gelatin with various spinning volumes to control the immobilized heparin content, which was ultimately intended to increase the immobilization efficiency of bFGF. The amount of bFGF on the heparin conjugated fibrous matrices depended on the thicknesses of the swollen matrices ranging from 35.4 ± 6.5 to 162.3 ± 14.0 ng and ≈90% of the bFGF was gradually released over a period of up to 56 d. The released bFGF enhanced the proliferation of human umbilical vein endothelial cells and human mesenchymal stem cells. In conclusion, our heparin‐conjugated fibrous matrices have the potential to be used as a growth factor delivery system in tissue engineering applications. magnified image

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