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โœฆ   LIBER   โœฆ

Relaxing effect of interleukin-1 on rat cultured Ito cells

โœ Scribed by M Sakamoto; T Ueno; H Sugawara; T Torimura; R Tsuji; K Sujaku; M Sata; K Tanikawa

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
691 KB
Volume
25
Category
Article
ISSN
0270-9139

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โœฆ Synopsis

Measurement of cGMP.

Culture media containing various concentrations of IL-1b (0-1,000 pmol/L) were prepared using serum-free Abbreviations: IL-1b, interleukin-1b; TNF-a, tumor necrosis factor a; cGMP; cyclic gua-WE and added to Ito cells cultured for 24 hours, and the intracellular nosine 3,5-monophosphate; WE, William's medium E; L-NMMA, N G -monomethyl-L-argi-cGMP concentration was determined after 12 hours by the following nine; SNAP, S-nitroso-N-acethyl-DL-penicillamine; iNOS, inducible nitric oxide; PBS, phosphate-buffered saline; FITC, fluorescein isothiocyanate. method. The supernatant of a culture of Ito cells was removed, and

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