✦ LIBER ✦
Relaxation spectra of two-step mechanisms as measured by the nadh-binding to glutamate dehydrogenase
✍ Scribed by Manfred Kempfle
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 638 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1522-9602
No coin nor oath required. For personal study only.
✦ Synopsis
Temperature-jump relaxation experiments performed with glutamate dehydrogenase and the reduced coenzyme nicotinamide adenosine dinueleotide show two clearly separated relaxation times. Three of the simplest and most plausible mechanisms attributable to two relaxation times are treated here theoretically and the concentration dependence of the relaxation times is determined in each case with different experimental conditions. This makes it possible to distinguish between the mechanisms and also to determine the rate constants of the different reaction steps.