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Relative effectiveness of various anions on the solubility of acidic Hypoderma lineatum collagenase at pH 7.2

โœ Scribed by Claire Carbonnaux; Madeleine Ries-Kautt; Arnaud Ducruix


Book ID
105356341
Publisher
Cold Spring Harbor Laboratory Press
Year
1995
Tongue
English
Weight
553 KB
Volume
4
Category
Article
ISSN
0961-8368

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โœฆ Synopsis


Abstract

The effects of various anions on decreasing the solubility of acidic Hypoderma lineatum collagenase at pH 7.2 and 18 ยฐC were qualitatively defined by replacing the crystallizing agent of known crystallization conditions by various ammonium salts. The solubility curves measured in the presence of the sulfate, phosphate, citrate, and chloride ammonium salts gave the following ranking of anions: HPO~4~^2โ€^/H~2~PO~4~^โˆ’^ > SO~4~^2โ€^ > citrate^3โ€^/citrate^2โ€^ >> Cl^โˆ’^. This order is in agreement with the Hofmeister series. In a previous study on the solubility at pH 4.5 of lysozyme, a basic protein, the effectiveness of anions in decreasing the solubility was found to be in the reverse order. This suggests that the effectiveness of anions in the crystallization of proteins is dependent on the net charge of the protein, i.e., depending on whether a basic protein is crystallized at acidic pH or an acidic protein at basic pH.


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