Relationships between the reversed-phase liquid chromatographic retention characteristics and physico-chemical parameters of some β-casomorphin peptides

✍ Scribed by Franka Kálmán; Tibor Cserháti; Klára Valkó; Klaus Neubert

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 638 KB
• Volume: 268
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85218-u

✦ Synopsis

Log kk and slope values for ten B-casomorphm peptldes were calculated m ten different reversed-phase hqmd chromatographlc systems usmg methanol as organic modifier The differences between the retention behavlours of C,, C,, and C, columns were fairly small the eluent composltlon had a greater Impact than the length of the hydrophobic hgand on the retention of peptldes A quaternary ammo column showed considerably different retention characterlstlcs The results suggest that each substructure m the peptldes makes a slmdar contnbutlon to the overall retention of the peptlde The retention parameters of peptldes mainly correlated with their electronic and stenc characterlstlcs This fmdmg emphasizes the importance of the electrostatic mteractlons between the free sdanol groups on the surface of the reversed-phase support and the polar substructures of peptldes Keywords Liquid chromatography, Casomorphm peptides, Peptldes