Relationships between the back-scatter of polarised light and the fibre-optic detection of connective tissue fluorescence in beef
✍ Scribed by Swatland, H J
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 176 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
The back-scatter of light (400È800 nm) from bovine m longissimus lumborum (n \ 47) was measured with a Ðbre-optic probe Ðtted with crossed polarisers to exclude Fresnel reÑectance. Unlike normal Ðbre-optic spectra (which may be relatively Ñat), back-scatter was approximately proportional to wavelength, being low at 400 nm and high at 800 nm. The shape of the spectrum was modi-Ðed by myoglobin absorbance, with a Soret minimum at 430 nm. Connective tissue Ñuorescence (365 nm excitation, 400È550 nm emission) was measured through a single optical Ðbre moving down the longitudinal axis of the muscle. Back-scatter at 430 nm was correlated positively with minimum Ñuorescence (r \ 0É73, P \ 0É001), the area under the Ñuorescence signal cm~1 (r \ 0É81, P \ 0É001) and Ñuorescence peaks cm~1 (r \ 0É46, P \ 0É005). Back-scatter at 800 nm was correlated weakly and negatively with minimum Ñuorescence (r \ [0É28, P \ 0É05) and peaks cm~1 (r \ [0É26, P \ 0É05). Thus, in the probe detection of connective tissue Ñuorescence in meat, errors caused by di †erences in myoglobin concentration may exceed those caused by di †erences in pH-related light scattering.