𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Relationships between nitrogenase, glutamine synthetase, glutamine, and energy charge inAzotobacter vinelandii

✍ Scribed by J. A. Kleinschmidt; D. Kleiner

Book ID
104760375
Publisher
Springer
Year
1981
Tongue
English
Weight
437 KB
Volume
128
Category
Article
ISSN
0302-8933

No coin nor oath required. For personal study only.

✦ Synopsis

When continuous cultures of Azotobacter vinelandii were supplied with ammonium or nitrate in amounts, which just repressed nitrogenase synthesis completely, both the intracellular glutamine level and the degree of adenylylation of the glutamine synthetase (GS) increased only slightly (from 0.45 -0.50 mM and from 2 to 3 respectively), while the total GS level remained unaffected. Higher amounts of ammonium additionally inhibited the nitrogenase activity, caused a strong rise in the intracellular glutamine concentration and adenylylation of the GS, but caused no change in the ATP/ADP ratio. These results are considered as evidence that in A. vinelandii the regulation ofnitrogenase synthesis is not linked to the adenylylation state of the GS and to the intracellular glutamine level, and that the inhibition of the nitrogenase activity as a consequence of a high extracellular ammonium level is not mediated via a change in the energy charge.

πŸ“œ SIMILAR VOLUMES

Localization and activities of nitrogena
Localization and activities of nitrogenase, glutamine synthetase and glutamate synthase inAzotobacter vinelandiigrown in oxygen-controlled continuous culture
✍ D. RΓΆckel; J. J. Hernando; E. Vakalopoulou; E. Post; J. Oelze πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 529 KB

Azotobacter vinelandii was grown in oxygen-controlled continuous cultures under conditions of dinitrogen fixation. Different oxygen concentrations were adjusted with air. Cell-free extracts were employed to study the oxygen dependency of the intracellular distribution and activity of the following e

The relationship between the state of ad
The relationship between the state of adenylylation of glutamine synthetase I and the export of ammonium in free-living, N2-fixingRhizobium
✍ William R. Evans; Deborah K. Crist πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 563 KB

The relationship between ammonium assimilation and ammonium export has been studied in free-living, Nzfixing Rhizobium sp. 32H1. After 55 to 67 h of microaerobic growth under a gas phase of 0.2% O2 -1.0% COz -98.8% Ar high levels of nitrogenase were observed concomitant with a slightly adenylylated