Relationship between the EBV-associated membrane antigen on burkitt lymphoma cells and the viral envelope, demonstrated by immunoferritin labelling

✍ Scribed by D. Silvestre; F. M. Kourilsky; G. Klein; Y. Yata; C. Neauport-Sautes; J. P. Levy

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: French
• Weight: 911 KB
• Volume: 8
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910080206

✦ Synopsis

Abstract

The localization of EBV‐induced, cell membrane antigens (MA) and intracellular viral capsid antigens (VCA) was studied by immunoferritin labelling. Human sera with known concordant or discordant anti‐MA and anti‐ VCA reactivities were allowed to react with intact, viable EBV‐carrier cells, and with naked and enveloped EBV‐particles. The reaction was visualized by the indirect immunoferritin test, using a rabbit anti‐human immunoglobulin conjugate. The following conclusions were drawn:

MA is a structural constituent of the viral envelope. Anti‐MA positive sera gave a consistently strong labelling of viral envelopes and of virus‐free patches on the cell membrane as well.

VCA is not present on the viral envelope and on the cell membrane. It is expressed on the surface of naked virus particles.

A multispecific anti‐HL.A serum gave a faint labelling with a small proportion of the enveloped viral particles. It could not be resolved whether this was due to the presence of HL.A antigens on some viral envelopes or, alternatively, to low anti‐MA levels in the anti‐HL.A sera.