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Relationship between membrane potential and external potassium in human glioblastoma cells in tissue culture

✍ Scribed by Laura Manuelidis; Elias E. Manuelidis; J. Prichard

Publisher: John Wiley and Sons
Year: 1976
Tongue: English
Weight: 830 KB
Volume: 87
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040870206

No coin nor oath required. For personal study only.

✦ Synopsis

Cells from a human glioblastoma (TC 526) maintained in tissue culture for ten years had a mean membrane potential of 27 +/- 0.9 mV at an external potassium concentration [Ko] of 5.3 mM. When [Ko] was varied between 2.5 and 5.3 mM, membrane potential changes were close to those predicted by the Nernst equation. At higher [Ko], the Nernstian slope was approached only in the presence of 10(-5) M ouabain, which did not affect membrane potential at a [Ko] of 5.3 mM. An electrogenic sodium pump activated by high [Ko] could explain these findings; such a mechanism has been demonstrated in other tissues.

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