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Relationship between cellular ATP content and cellular functions of primary cultured rat hepatocytes in hypoxia

✍ Scribed by KATSUHIRO HAYASHI; TOSHIMASA OCHIAI; YOSHIHIRO ISHINODA; TAKESHI OKAMOTO; TOSHIHIRO MARUYAMA; KAZUNORI TSUDA; HIROHITO TSUBOUCHI


Book ID
108949424
Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
722 KB
Volume
12
Category
Article
ISSN
0815-9319

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Primary hepatocytes were cultured at oxygen tensions similar to those reported to be present in periportal (13% 02) and pericentral (4% 0,) regions of the liver lobules. Cellular DNA and protein content of individual hepatocytes were determined simultaneously by two-parameter (DNAlprotein) flow cyto