Relationship between cell migration and cell cycle during the initiation of epithelial to fibroblastoid transition

✍ Scribed by Bonneton, C. ;Sibarita, J.-B. ;Thiery, J.-P.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 196 KB
• Volume: 43
• Category: Article
• ISSN: 0886-1544
• DOI: 10.1002/(sici)1097-0169(1999)43:4<288::aid-cm2>3.0.co;2-y

✦ Synopsis

The NBT-II rat bladder carcinoma cell line, which displays epithelial to mesenchymal transition or EMT in response to FGF-1 stimulation, was used to study the interrelationships between cell cycle and cell scattering and locomotion. Time-lapse video microscopy experiments were performed with asynchronous growing cells and lovastatin-arrested cells. FGF-1 stimulation induced cell movement in cells in all phases of the cell cycle, except G2 + M phase, in which cells did not respond to stimulation. The delay between cell stimulation and cell movement depended on the age of the cell at the beginning of cell stimulation: cells less than 4 h old when stimulated by FGF-1 had a 1-h delay whereas cells more than 4 h old had a 3-h delay. Cells stimulated before they were 4 h old were temporarily arrested in their cell cycle progression. Older cells underwent mitosis on schedule. Lovastatin-treated cells were shown to be synchronized in the G1 phase and to migrate simultaneously after FGF-1 stimulation. These results indicate that the G1 phase was a critical phase for FGF-1 induced cell migration during epithelial to fibroblastoid transition.