Catharanthus roseus hairy root cultures, genetically transformed with Agrobacterium rhizogenes, produce a wide variety of indole alkaloids. The effect of sucrose, phosphate, nitrate, and ammonia concentrations on growth and indole alkaloid production of C. roseus hairy root cultures were studied by
Relation between the amount of rolC gene product and indole alkaloid accumulation in Catharanthus roseus transformed root cultures
✍ Scribed by Javier Palazón; Rosa M. Cusidó; Jordi Gonzalo; Mercedes Bonfill; Carmen Morales; M.Teresa Piñol
- Book ID
- 117392599
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 838 KB
- Volume
- 153
- Category
- Article
- ISSN
- 0176-1617
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## Abstract Nitric oxide (NO) is known as a signaling molecule involved in elicitor‐induced defense responses of plants. Sodium nitroprusside (SNP), a donor of NO, stimulates catharanthine formation in Catharanthus roseus cells.1 Two important terpenoid indole alkaloids produced in small quantities
The kinetics of growth, the uptake of macronutrients, and the accumulation of indole alkaloids were investigated in long-term, heterotrophically cultured transgenic (''hairy'') roots of Catharanthus roseus. Tabersonine, ajmalicine, and serpentine were monitored over a 70-day period. The doubling tim