Relation between protective potency and specificity of antiiiodies in sera of tetanus immunized individuals

✍ Scribed by M. German-Fattal; A. German; B. Bizzini

• Book ID: 104678157
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 392 KB
• Volume: 3
• Category: Article
• ISSN: 0393-2990
• DOI: 10.1007/bf00239747

✦ Synopsis

Out of 157 human sera analyzed for antitetanus antibody content by ELISA, 13 turned out to contain only anti-BIIb antibodies, of which 8 proved to be neutralizing. Of these, the 3 sera 303, 306 and 312 together with a commercially available standard preparation of human antitetanus immunoglobulins were further analyzed as to their antibody composition by ELISA using plates sensitized with either the toxoid or various tetanus toxin-derived fragments. It was verified that the protective potency of these antisera was related mainly to their anti-BIIb antibody content. Adsorption experiments confirmed that anti-BIIb antibodies were primarily involved in toxin neutralization, although the presence of high levels of both anti-alpha and anti-Ibc antibodies could confer neutralizing capacity on the sera. A rabbit antiserum raised with the BIIb fragment resulted in a neutralizing antiserum that allowed us to calibrate the ELISA with the anti-BIIb antibodies as International Units.