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Reiterated DNA sequences in a mutant strain of Streptomyces glaucescens and cloning of the sequence in Escherichia coli

✍ Scribed by Ono, Hideo ;Hintermann, Gilberto ;Crameri, Reto ;Wallis, Gillian ;Hütter, Ralf

Publisher
Springer
Year
1982
Tongue
English
Weight
581 KB
Volume
186
Category
Article
ISSN
0026-8925

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✦ Synopsis

Streptomyces glaucescens exhibits a high degree of genetic instability. A sequence of 7.2 kb has been found which is present in a few tandemly repeated copies in the wild type strain GLA 0 and is amplified to ca. 500 copies per genome in the mutant strain GLA 1204. This sequence was cloned in Escherichia coli using pBR325 as vector.

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